Hotspot

trnC-GCA-petN

LSC · 30336-31183 · 848 bp

Back to Pterocarya

Pi 0.00338

MarkerSeek score 42.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 7
pi: 0.00338
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	938 bp	1.0	76.5
trnC-GCA-petN_p2	GTATCGTTTTGGCGACATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	972 bp	1.0	75.7
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	913 bp	1.0	75.6
trnC-GCA-petN_p4	GGTGTCGCCTGATCAACAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.8	58.2	901 bp	1.0	73.3
trnC-GCA-petN_p5	TTCAAATCTGGGTGTCGCC	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	911 bp	1.0	70.9