Hotspot

rps16-trnQ-UUG

LSC · 6460-8012 · 1553 bp

Back to Pterocarya

Pi 0.00342

MarkerSeek score 60.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1553
variable_sites: 14
pi: 0.00342
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AATGGCGGATGTCAGAATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1626-1674 bp	1.0	83.1
rps16-trnQ-UUG_p2	TCCACAACCGACCATATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1609-1657 bp	1.0	82.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1587-1635 bp	1.0	82.2
rps16-trnQ-UUG_p4	AATGGCGGATGTCAGAATCC	TGGCTAAGTGGTAAGGCAAC	50.0	50.0	58.0	57.8	1667-1715 bp	1.0	82.0
rps16-trnQ-UUG_p5	CAGAATCCACAACCGACCAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1614-1662 bp	0.9	78.4