Hotspot

trnK-UUU-rps16

LSC · 4240-5296 · 1057 bp

Back to Pterocarya

Pi 0.00341

MarkerSeek score 56.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 11
pi: 0.00341
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGTCGCGGTCTTACAAAG	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	57.9	57.6	1235-1239 bp	1.0	77.8
trnK-UUU-rps16_p2	TCAGTCGCGGTCTTACAAAG	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	57.9	57.6	1236-1240 bp	1.0	77.7
trnK-UUU-rps16_p3	TCAGTCGCGGTCTTACAAAG	TTTCAAAGAAGGCGGGAGTT	50.0	45.0	57.9	57.6	1237-1241 bp	1.0	77.7
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	58.2	57.6	1148-1152 bp	1.0	77.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	58.2	57.6	1149-1153 bp	1.0	77.0