Hotspot

trnH-GUG-psbA

LSC · 117-302 · 186 bp

Back to Pterocarya

Pi 0.043

MarkerSeek score 67.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 40
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	303 bp	0.9	75.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	317 bp	0.9	74.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	316 bp	0.9	74.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	318 bp	0.9	74.9
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.5	58.0	332 bp	0.9	72.1