Hotspot

ndhG-ndHI

SSC · 122899-123541 · 643 bp

Back to Pterocarpus

Pi 0.0544

MarkerSeek score 80.40

Primer available yes

Label ndhG-ndHI

Feature Metadata

label_name: ndhG-ndHI
feature_type: igs
region: SSC
length_bp: 643
variable_sites: 85
pi: 0.0544
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndHI_p1	AGTGGTTCAGGCAAATCCAT	AGGCCGTTTACCAATGTCAG	45.0	50.0	57.4	58.2	352-729 bp	1.0	85.7
ndhG-ndHI_p2	AGTGGTTCAGGCAAATCCAT	TGCTTTAGGCCGTTTACCAA	45.0	45.0	57.4	57.7	358-735 bp	1.0	84.9
ndhG-ndHI_p3	AGTGGTTCAGGCAAATCCAT	TTGCTTTAGGCCGTTTACCA	45.0	45.0	57.4	57.7	359-736 bp	1.0	84.9
ndhG-ndHI_p4	AGTGGTTCAGGCAAATCCAT	GGCCGTTTACCAATGTCAGT	45.0	50.0	57.4	58.5	351-728 bp	1.0	83.4
ndhG-ndHI_p5	GTAGTGGTTCAGGCAAATCCA	AGGCCGTTTACCAATGTCAG	47.6	50.0	58.2	58.2	354-731 bp	1.0	80.9