Hotspot

ndhE-ndhG

SSC · 122130-122367 · 238 bp

Back to Pterocarpus

Pi 0.043

MarkerSeek score 79.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 238
variable_sites: 23
pi: 0.043
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACAAGTACATGTTCGAGCATCA	AGCTGCTTTGATAGGTGCAA	40.9	45.0	58.1	57.8	292-303 bp	1.0	71.9
ndhE-ndhG_p2	GGCACTCAAAACAAGTACATGT	AGCTGCTTTGATAGGTGCAA	40.9	45.0	57.4	57.8	302-313 bp	1.0	67.8
ndhE-ndhG_p3	CAAGTACATGTTCGAGCATCA	AGCTGCTTTGATAGGTGCAA	42.9	45.0	56.4	57.8	291-302 bp	1.0	67.5
ndhE-ndhG_p4	ACAAGTACATGTTCGAGCATC	AGCTGCTTTGATAGGTGCAA	42.9	45.0	56.4	57.8	292-303 bp	1.0	67.4
ndhE-ndhG_p5	AACAAGTACATGTTCGAGCA	AGCTGCTTTGATAGGTGCAA	40.0	45.0	55.3	57.8	293-304 bp	1.0	67.1