Hotspot

trnL-UAG-ccsA

SSC · 118309-118407 · 99 bp

Back to Pterocarpus

Pi 0.0281
MarkerSeek score 65.60
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
99
variable_sites
9
pi
0.0281
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ACAATTGATACGACCGAAAACG 50.0 40.9 57.9 57.7 221-249 bp 1.0 65.4
trnL-UAG-ccsA_p2 GTAGACACGCTGCTCTTAGG ACAATTGATACGACCGAAAACG 55.0 40.9 57.8 57.7 222-250 bp 1.0 64.8
trnL-UAG-ccsA_p3 AAATTGGTAGACACGCTGCT ACAATTGATACGACCGAAAACG 45.0 40.9 57.8 57.7 228-256 bp 1.0 64.7
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG ACAATTGATACGACCGAAAACG 55.0 40.9 57.8 57.7 223-251 bp 1.0 64.7
trnL-UAG-ccsA_p5 AATTGGTAGACACGCTGCTC ACAATTGATACGACCGAAAACG 50.0 40.9 58.3 57.7 227-255 bp 1.0 64.0