Hotspot

rps8-rpl14

LSC · 84414-84793 · 380 bp

Back to Pterocarpus

Pi 0.0317

MarkerSeek score 71.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 32
pi: 0.0317
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	421-463 bp	1.0	86.6
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.4	57.9	417-459 bp	1.0	86.3
rps8-rpl14_p3	TATGTCAGCGATGGTGTCCT	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.5	57.9	420-462 bp	1.0	85.0
rps8-rpl14_p4	TTATGTCAGCGATGGTGTCC	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.7	57.6	422-464 bp	1.0	84.2
rps8-rpl14_p5	GTCAGCGATGGTGTCCTTAC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.4	57.6	418-460 bp	1.0	83.9