Hotspot

rps11-rpl36

LSC · 82883-83450 · 568 bp

Back to Pterocarpus

Pi 0.0248

MarkerSeek score 65.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 36
pi: 0.0248
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	CCAAGACATAAACAAAGACAAGGA	50.0	37.5	58.1	57.4	592-643 bp	0.555556	32.2
rps11-rpl36_p2	CGTCCATTTTTACGCGAACC	TCCAAGACATAAACAAAGACAAGG	50.0	37.5	58.1	57.4	593-644 bp	0.555556	32.2
rps11-rpl36_p3	CGTCCATTTTTACGCGAACC	TGTTCCAATCCAAGACATAAACA	50.0	34.8	58.1	56.4	601-652 bp	0.555556	32.1
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	CGAGGGCGAATTATAGTAATTTGT	50.0	37.5	58.1	57.2	622-673 bp	0.555556	30.8
rps11-rpl36_p5	TTACGCGAACCAATACGTCC	CCAAGACATAAACAAAGACAAGGA	50.0	37.5	58.4	57.4	607-658 bp	0.555556	30.2