Hotspot

clpP-psbB

LSC · 75294-75781 · 488 bp

Back to Pterocarpus

Pi 0.0277

MarkerSeek score 66.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 31
pi: 0.0277
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CCAGGACTTCGAAAGGGAAC	GCGGCCGGGATCATTTAATA	55.0	50.0	58.2	58.2	557-583 bp	1.0	85.4
clpP-psbB_p2	CCAGGACTTCGAAAGGGAAC	CGATACCAAGGCAAACCCAT	55.0	50.0	58.2	58.2	523-549 bp	1.0	85.3
clpP-psbB_p3	CCAGGACTTCGAAAGGGAAC	ATGGACAGAAAGTAAGCGGC	55.0	50.0	58.2	58.3	572-598 bp	1.0	84.7
clpP-psbB_p4	TCCAGGACTTCGAAAGGGAA	GCGGCCGGGATCATTTAATA	50.0	50.0	58.3	58.2	558-584 bp	1.0	84.7
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	GCGGCCGGGATCATTTAATA	50.0	50.0	57.7	58.2	563-589 bp	1.0	84.4