Hotspot

trnP-UGG-psaJ

LSC · 69501-70123 · 623 bp

Back to Pterocarpus

Pi 0.0188

MarkerSeek score 68.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 29
pi: 0.0188
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	719-729 bp	1.0	83.9
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	718-728 bp	1.0	83.9
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	730-740 bp	1.0	83.8
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	729-739 bp	1.0	83.8
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	732-742 bp	1.0	83.7