Hotspot

psbE-petL

LSC · 67291-68658 · 1368 bp

Back to Pterocarpus

Pi 0.00949

MarkerSeek score 64.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1368
variable_sites: 42
pi: 0.00949
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1438-1564 bp	1.0	86.1
psbE-petL_p2	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1440-1566 bp	1.0	86.0
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1427-1553 bp	1.0	85.8
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1429-1555 bp	1.0	85.7
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.2	58.0	1429-1555 bp	1.0	84.3