Hotspot

accD-psaI

LSC · 61991-62537 · 547 bp

Back to Pterocarpus

Pi 0.0205

MarkerSeek score 65.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 34
pi: 0.0205
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	687-714 bp	1.0	87.9
accD-psaI_p2	AAGGTTCACAAGCAGCTGAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	729-756 bp	1.0	87.2
accD-psaI_p3	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	791-818 bp	1.0	87.0
accD-psaI_p4	CTACTACTGGTGGGGTGACA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	855-882 bp	1.0	86.0
accD-psaI_p5	TACTACTGGTGGGGTGACAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	854-881 bp	1.0	86.0