Hotspot

trnV-UAC-ndhC

LSC · 10324-10861 · 538 bp

Back to Pterocarpus

Pi 0.0227

MarkerSeek score 64.60

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 30
pi: 0.0227
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	GCTGGATATCATTAGAAATGCCC	50.0	43.5	57.8	57.4	395-625 bp	1.0	58.1
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	GGCTGGATATCATTAGAAATGCC	50.0	43.5	57.8	57.4	396-626 bp	1.0	58.0
trnV-UAC-ndhC_p3	GAAGGTCTACGGTTCGAGTC	GCTGGATATCATTAGAAATGCCC	55.0	43.5	57.5	57.4	394-624 bp	1.0	55.3
trnV-UAC-ndhC_p4	GAAGGTCTACGGTTCGAGTC	GGCTGGATATCATTAGAAATGCC	55.0	43.5	57.5	57.4	395-625 bp	1.0	55.3
trnV-UAC-ndhC_p5	GTTCGAGTCCGTATAGCCCT	GCTGGATATCATTAGAAATGCCC	55.0	43.5	58.7	57.4	383-613 bp	1.0	54.3