Hotspot

ycf1

SSC · 125831-131236 · 5406 bp

Back to Pternopetalum

Pi 0.0118

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5406
variable_sites: 64
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACAAATTCTGGGAAGAACGT	CCTGGACCAAAATGGATTATAACA	42.9	37.5	56.7	57.0	233 bp	1.0	43.1
ycf1_p2	CACAAATTCTGGGAAGAACGT	CCTGGACCAAAATGGATTATAACAG	42.9	40.0	56.7	58.0	233 bp	1.0	43.1
ycf1_p3	CACAAATTCTGGGAAGAACGT	CCTGGACCAAAATGGATTAT	42.9	40.0	56.7	52.8	233 bp	1.0	43.1
ycf1_p4	ACAAATTCTGGGAAGAACGT	CCTGGACCAAAATGGATTATAACA	40.0	37.5	55.2	57.0	232 bp	1.0	43.1
ycf1_p5	ACAAATTCTGGGAAGAACGT	CCTGGACCAAAATGGATTATAACAG	40.0	40.0	55.2	58.0	232 bp	1.0	43.1