Hotspot

ccsA

SSC · 116384-117358 · 975 bp

Back to Pternopetalum

Pi 0.00718

MarkerSeek score 48.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 7
pi: 0.00718
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AACCGACAAAAGCCTGGAAT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1922-1936 bp	1.0	80.9
ccsA_p2	ACCGACAAAAGCCTGGAATT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1921-1935 bp	1.0	80.9
ccsA_p3	AACCGACAAAAGCCTGGAAT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1921-1935 bp	1.0	80.9
ccsA_p4	ACCGACAAAAGCCTGGAATT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1920-1934 bp	1.0	80.9
ccsA_p5	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	1504-1510 bp	1.0	80.9