Hotspot

clpP-psbB

LSC · 72725-73184 · 460 bp

Back to Pternopetalum

Pi 0.011

MarkerSeek score 63.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 5
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GCACTATACGTCAACCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	549-555 bp	1.0	79.2
clpP-psbB_p2	CAGGACTTCGAAAAGGCACT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	514-520 bp	1.0	78.9
clpP-psbB_p3	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	45.0	50.0	58.1	58.2	501-507 bp	1.0	78.7
clpP-psbB_p4	GTCAACCCAGGATGCATCTT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	540-546 bp	1.0	78.6
clpP-psbB_p5	GCACTATACGTCAACCCAGG	ATATGGACAGACAGCAACCG	55.0	50.0	58.1	57.7	600-606 bp	1.0	78.4