Hotspot

ndhK-ndhC

LSC · 51008-51056 · 49 bp

Back to Pternopetalum

Pi 0.0408

MarkerSeek score 65.70

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 49
variable_sites: 2
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGATCAAGT	AAAAGGGGCATTGGAATGGT	40.0	45.0	55.5	58.0	117 bp	1.0	60.9
ndhK-ndhC_p2	GGGTTGTTCGATCAAGTAAAGG	AAAAGGGGCATTGGAATGGT	45.5	45.0	57.3	58.0	114 bp	1.0	59.6
ndhK-ndhC_p3	TTTTGGGTTGTTCGATCAAGT	AAAAGGGGCATTGGAATGGT	38.1	45.0	56.3	58.0	118 bp	1.0	59.3
ndhK-ndhC_p4	ACTGAATTTTGGGTTGTTCGA	AAAAGGGGCATTGGAATGGT	38.1	45.0	56.3	58.0	124 bp	1.0	59.2
ndhK-ndhC_p5	TTTGGGTTGTTCGATCAAGT	CGAAAAGGGGCATTGGAATG	40.0	50.0	55.5	57.7	119 bp	1.0	58.9