Hotspot

psbK-psbI

LSC · 7709-8132 · 424 bp

Back to Pternopetalum

Pi 0.0251

MarkerSeek score 63.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 10
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAAAGTTTGAGAGTAAGCA	50.0	36.4	57.9	56.2	452-477 bp	1.0	55.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	449-474 bp	1.0	53.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	450-475 bp	1.0	53.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	451-476 bp	1.0	53.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAAAAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.9	453-478 bp	1.0	52.8