Hotspot

ycf1

SSC · 127024-132660 · 5637 bp

Back to Pternandra

Pi 0.0142

MarkerSeek score 46.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 80
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCTTGCGAAAAGGAGGATA	ACGGACCTTCCTTTTGGTTC	50.0	50.0	58.0	58.0	855 bp	1.0	82.1
ycf1_p2	TCCGATTCCTGATTGCGTTT	ACGGACCTTCCTTTTGGTTC	45.0	50.0	57.9	58.0	427 bp	1.0	81.4
ycf1_p3	GGCTTGCGAAAAGGAGGATA	CGGAAGCATGGGATAGCATT	50.0	50.0	58.0	58.1	1244 bp	1.0	81.0
ycf1_p4	ATAGGCTTGCGAAAAGGAGG	ACGGACCTTCCTTTTGGTTC	50.0	50.0	58.0	58.0	858 bp	0.5	59.5
ycf1_p5	ATAGGCTTGCGAAAAGGAGG	CGGAAGCATGGGATAGCATT	50.0	50.0	58.0	58.1	1247 bp	0.5	58.9