Hotspot

ndhG-ndhI

SSC · 121791-122248 · 458 bp

Back to Pternandra

Pi 0.0316

MarkerSeek score 63.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 458
variable_sites: 14
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCATTCCCAACAGTCCAAA	GGGCAACAAACAATACGAGC	50.0	50.0	58.0	58.0	1223-1238 bp	1.0	82.0
ndhG-ndhI_p2	CCCATTCCCAACAGTCCAAA	CAAACAATACGAGCGGCAAG	50.0	50.0	58.0	58.1	1217-1232 bp	1.0	81.3
ndhG-ndhI_p3	CCCATTCCCAACAGTCCAAA	CCGCTTATGATCGTCACGAA	50.0	50.0	58.0	58.2	874-889 bp	1.0	81.3
ndhG-ndhI_p4	CCCATTCCCAACAGTCCAAA	ACATTGGGCAAGGATTCCTG	50.0	50.0	58.0	58.1	1195 bp	0.5	58.8
ndhG-ndhI_p5	CCCATTCCCAACAGTCCAAA	CATTGGGCAAGGATTCCTGA	50.0	50.0	58.0	57.9	1194 bp	0.5	58.7