Hotspot

ndhD-psaC

SSC · 120099-120249 · 151 bp

Back to Pternandra

Pi 0.0662

MarkerSeek score 76.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 151
variable_sites: 10
pi: 0.0662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAAATTAAAGAACCCGCCA	AAACAACTCGAAGCATGGGT	38.1	45.0	56.0	58.0	250 bp	1.0	59.4
ndhD-psaC_p2	AGAAAATTAAAGAACCCGCCA	GGCATGAAACAACTCGAAGC	38.1	50.0	56.0	58.0	256 bp	1.0	59.2
ndhD-psaC_p3	AAGAAAATTAAAGAACCCGCCA	AAACAACTCGAAGCATGGGT	36.4	45.0	56.8	58.0	251 bp	1.0	57.3
ndhD-psaC_p4	AGAAAATTAAAGAACCCGCCAA	AAACAACTCGAAGCATGGGT	36.4	45.0	56.8	58.0	250 bp	1.0	57.3
ndhD-psaC_p5	AGAAAATTAAAGAACCCGCCAA	GGCATGAAACAACTCGAAGC	36.4	50.0	56.8	58.0	256 bp	1.0	57.2