Hotspot

ccsA-ndhD

SSC · 118304-118589 · 286 bp

Back to Pternandra

Pi 0.0524
MarkerSeek score 72.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
286
variable_sites
15
pi
0.0524
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGTCA TCAGTTGAGAAGGTCGAAGC 40.9 50.0 57.2 57.6 406 bp 1.0 57.0
ccsA-ndhD_p2 TGGATATGCTATTTTGGGGTCA CAGTTGAGAAGGTCGAAGCT 40.9 50.0 57.2 57.6 405 bp 1.0 56.9
ccsA-ndhD_p3 TGGATATGCTATTTTGGGGTCAA TCAGTTGAGAAGGTCGAAGC 39.1 50.0 57.9 57.6 406 bp 1.0 54.3
ccsA-ndhD_p4 TTGGATATGCTATTTTGGGGTCA TCAGTTGAGAAGGTCGAAGC 39.1 50.0 57.9 57.6 407 bp 1.0 54.3
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGTCAA CAGTTGAGAAGGTCGAAGCT 39.1 50.0 57.9 57.6 405 bp 1.0 54.3