Hotspot

ndhF-rpl32

SSC · 115679-116706 · 1028 bp

Back to Pternandra

Pi 0.0358

MarkerSeek score 73.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1028
variable_sites: 35
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AACGAAAAAGCCTTCAACGC	50.0	45.0	58.0	58.2	1137-1181 bp	1.0	84.2
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	GCCTTCAACGCTGCAAAATA	50.0	45.0	58.0	57.7	1128-1172 bp	1.0	82.8
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	CGAAAAAGCCTTCAACGCTG	50.0	50.0	58.0	58.6	1135-1179 bp	1.0	80.7
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAACGCTGC	50.0	50.0	58.0	58.9	1134-1178 bp	1.0	78.6
ndhF-rpl32_p5	TGGGAAGTGGAATGAGAGGT	CGCTAACGAAAAAGCCTTCA	50.0	45.0	58.0	57.0	1141-1185 bp	1.0	77.8