Hotspot

psbT-psbN

LSC · 76499-76573 · 75 bp

Back to Pternandra

Pi 0.133

MarkerSeek score 87.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 10
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCGCCGAAAGTTCCAACTAA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.5	58.0	134 bp	1.0	81.9
psbT-psbN_p2	CCGCCGAAAGTTCCAACTAA	TTCGAAGAACACGGGGACTA	50.0	50.0	58.5	58.4	129 bp	1.0	79.3
psbT-psbN_p3	CCGCCGAAAGTTCCAACTA	ATCCATTCGAAGAACACGGG	52.6	50.0	57.8	58.0	134 bp	1.0	76.0
psbT-psbN_p4	CCGCCGAAAGTTCCAACTAA	TCGAAGAACACGGGGACTAG	50.0	55.0	58.5	58.8	128 bp	1.0	75.7
psbT-psbN_p5	CGCCGAAAGTTCCAACTAAA	ATCCATTCGAAGAACACGGG	45.0	50.0	56.7	58.0	133 bp	1.0	75.2