Hotspot

ndhC-trnV-UAC

LSC · 52516-52973 · 458 bp

Back to Pternandra

Pi 0.0306
MarkerSeek score 63.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
458
variable_sites
14
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATACTCA AGAAGGTCTACGGTTCGAGT 36.4 50.0 54.9 57.8 527-532 bp 1.0 42.3
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATACTCA GAAGGTCTACGGTTCGAGTC 36.4 55.0 54.9 57.5 526-531 bp 1.0 42.3
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATACTCAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 55.5 57.8 527-532 bp 1.0 42.3
ndhC-trnV-UAC_p4 TGCCCAGAAAATATCATACTCA AAGGCATGTTGGGTCTTTGA 36.4 45.0 54.9 57.6 594-599 bp 1.0 42.0
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATACTCA AGGCATGTTGGGTCTTTGAA 36.4 45.0 54.9 57.6 593-598 bp 1.0 42.0