Hotspot

trnC-GCA-ndhJ

LSC · 49914-50783 · 870 bp

Back to Pternandra

Pi 0.026

MarkerSeek score 67.10

Primer available yes

Label trnC-GCA-ndhJ

Feature Metadata

label_name: trnC-GCA-ndhJ
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 22
pi: 0.026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	55.0	50.0	58.0	57.9	1047-1048 bp	1.0	83.0
trnC-GCA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	55.0	50.0	58.0	57.9	1046-1047 bp	1.0	83.0
trnC-GCA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	1011-1012 bp	1.0	82.9
trnC-GCA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	50.0	50.0	58.1	57.9	1011-1012 bp	1.0	82.7
trnC-GCA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	50.0	50.0	58.1	57.9	1010-1011 bp	1.0	82.7