Hotspot

psaA-ycf3

LSC · 43046-43634 · 589 bp

Back to Pternandra

Pi 0.0172

MarkerSeek score 69.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 10
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	AAGATCACAAGGCGTTTCGA	50.0	45.0	58.1	57.8	705-712 bp	1.0	79.8
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	AGATCACAAGGCGTTTCGAA	50.0	45.0	58.1	57.8	704-711 bp	1.0	79.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	686-693 bp	1.0	79.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	685-692 bp	1.0	79.7
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	TAGCGCTTACTCCCGGTAAT	50.0	50.0	58.1	58.3	749-756 bp	1.0	78.6