Hotspot

trnE-UUC-trnT-GGU

LSC · 31764-32687 · 924 bp

Back to Pternandra

Pi 0.0231

MarkerSeek score 61.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 20
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGGATCAGCGAATTCTTGGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1159-1211 bp	1.0	84.9
trnE-UUC-trnT-GGU_p2	TGGATCAGCGAATTCTTGGG	GACAGATTCGTGTGGAGTCC	50.0	55.0	58.0	58.0	1562-1618 bp	1.0	84.3
trnE-UUC-trnT-GGU_p3	TGGATCAGCGAATTCTTGGG	CAAGACTACGGAATCGGCAA	50.0	50.0	58.0	58.0	1586-1642 bp	1.0	84.2
trnE-UUC-trnT-GGU_p4	TGGATCAGCGAATTCTTGGG	AACAAGACTACGGAATCGGC	50.0	50.0	58.0	58.0	1588-1644 bp	1.0	84.2
trnE-UUC-trnT-GGU_p5	TTGATTTCGTCGGGACTGAC	GACAGATTCGTGTGGAGTCC	50.0	55.0	57.9	58.0	2053-2115 bp	1.0	83.6