Hotspot

trnR-UCU-atpA

LSC · 10242-10644 · 403 bp

Back to Pternandra

Pi 0.0649

MarkerSeek score 83.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 25
pi: 0.0649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CACCGAGGAAGCAGAATCTC	47.6	55.0	57.3	58.1	494-495 bp	1.0	72.9
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CATTCACCGAGGAAGCAGAA	47.6	50.0	57.3	57.9	498-499 bp	1.0	72.6
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AAGACATTCACCGAGGAAGC	47.6	50.0	57.3	57.9	502-503 bp	1.0	72.5
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCGAGGAAGCAGAATCTCT	47.6	50.0	57.3	58.1	493-494 bp	1.0	72.3
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TTCACCGAGGAAGCAGAATC	47.6	50.0	57.3	57.6	496-497 bp	1.0	70.3