Hotspot

rps16-trnQ-UUG

LSC · 6033-7272 · 1240 bp

Back to Pternandra

Pi 0.0259

MarkerSeek score 66.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1240
variable_sites: 32
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCGTCAAGTCACAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1324-1339 bp	1.0	80.5
rps16-trnQ-UUG_p2	ACACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	1308-1323 bp	1.0	79.1
rps16-trnQ-UUG_p3	TCACACGTTGCTTTCTACCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.4	58.1	1310-1325 bp	1.0	76.9
rps16-trnQ-UUG_p4	CCACAGATGATCATGTCCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	1334-1349 bp	1.0	76.8
rps16-trnQ-UUG_p5	CACACGTTGCTTTCTACCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.3	58.1	1309-1324 bp	1.0	76.4