Hotspot

rpl2

LSC · 79766-81331 · 1566 bp

Pi 0.104

MarkerSeek score 84.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1566
variable_sites: 452
pi: 0.104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CCCCGACCCGTGATTTATTT	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.0	58.0	2501-2509 bp	0.857143	82.5
rpl2_p2	CCCCGACCCGTGATTTATTT	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.0	58.0	2502-2510 bp	0.857143	82.5
rpl2_p3	AGATAGGCAGATGCTCCTGT	TGGCGAATTCACAGGTTCAA	50.0	45.0	57.9	58.0	2355-2363 bp	0.857143	82.1
rpl2_p4	AGATAGGCAGATGCTCCTGT	TTGGCGAATTCACAGGTTCA	50.0	45.0	57.9	58.0	2356-2364 bp	0.857143	82.1
rpl2_p5	CCCCGACCCGTGATTTATTT	AAGTGAGGGGGATACTACGG	50.0	55.0	58.0	57.9	2278-2287 bp	0.857143	81.8