Hotspot

rps12-rrn16

IRa · 142647-150037 · 7391 bp

Back to Pteris

Pi 0.267
MarkerSeek score 66.60
Primer available yes
Label rps12-rrn16

Feature Metadata

label_name
rps12-rrn16
feature_type
igs
region
IRa
length_bp
7391
variable_sites
3132
pi
0.267
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.52
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rrn16_p1 TAGTTAGCTTGTTCCGCGAC CGAACCGAAGAGGAACAGAG 50.0 55.0 58.0 58.0 1216-1301 bp 0.285714 63.2
rps12-rrn16_p2 TAGTTAGCTTGTTCCGCGAC CTTCTCTTTCTCACCCGCTC 50.0 55.0 58.0 58.0 1136 bp 0.142857 45.6
rps12-rrn16_p3 GTGAGATACCACCAAGCAGG CTTCTCTTTCTCACCCGCTC 55.0 55.0 58.0 58.0 899 bp 0.142857 45.5
rps12-rrn16_p4 ACCAAGCAGGGTTAGAGACT CTTCTCTTTCTCACCCGCTC 50.0 55.0 58.0 58.0 889 bp 0.142857 45.4
rps12-rrn16_p5 GCCTATAACAACCCGCTTGA CTTCTCTTTCTCACCCGCTC 50.0 55.0 58.0 58.0 792 bp 0.142857 45.4