Hotspot

rrn16-rps12

IRb · 92144-99534 · 7391 bp

Back to Pteris

Pi 0.267
MarkerSeek score 66.70
Primer available yes
Label rrn16-rps12

Feature Metadata

label_name
rrn16-rps12
feature_type
igs
region
IRb
length_bp
7391
variable_sites
3121
pi
0.267
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.53
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-rps12_p1 CGAACCGAAGAGGAACAGAG TAGTTAGCTTGTTCCGCGAC 55.0 50.0 58.0 58.0 1216-1301 bp 0.285714 63.2
rrn16-rps12_p2 CTTCTCTTTCTCACCCGCTC TAGTTAGCTTGTTCCGCGAC 55.0 50.0 58.0 58.0 1136 bp 0.142857 45.6
rrn16-rps12_p3 CTTCTCTTTCTCACCCGCTC GTGAGATACCACCAAGCAGG 55.0 55.0 58.0 58.0 899 bp 0.142857 45.5
rrn16-rps12_p4 CTTCTCTTTCTCACCCGCTC ACCAAGCAGGGTTAGAGACT 55.0 50.0 58.0 58.0 889 bp 0.142857 45.4
rrn16-rps12_p5 CTTCTCTTTCTCACCCGCTC GCCTATAACAACCCGCTTGA 55.0 50.0 58.0 58.0 792 bp 0.142857 45.4