Hotspot

rps4-trnL-CAA

LSC · 44013-44746 · 734 bp

Back to Pteris

Pi 0.141
MarkerSeek score 85.10
Primer available yes
Label rps4-trnL-CAA

Feature Metadata

label_name
rps4-trnL-CAA
feature_type
igs
region
LSC
length_bp
734
variable_sites
292
pi
0.141
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnL-CAA_p1 GCGTTCTGTCAATCCGTAGT ACGTCTCCATACCTAGGCTC 50.0 55.0 58.0 58.0 958-971 bp 0.857143 84.4
rps4-trnL-CAA_p2 GCGTTCTGTCAATCCGTAGT CGTCTCCATACCTAGGCTCA 50.0 55.0 58.0 58.0 957-970 bp 0.857143 84.4
rps4-trnL-CAA_p3 AACGCATCTCAAGCAATTGC ACGTCTCCATACCTAGGCTC 45.0 55.0 58.0 58.0 1040-1053 bp 0.857143 84.2
rps4-trnL-CAA_p4 AACGCATCTCAAGCAATTGC CGTCTCCATACCTAGGCTCA 45.0 55.0 58.0 58.0 1039-1052 bp 0.857143 84.2
rps4-trnL-CAA_p5 GCGTTCTGTCAATCCGTAGT TGAGGTGATGCGAGATTTGG 50.0 50.0 58.0 58.0 1037-1050 bp 0.571429 69.9