Hotspot

atpH-atpI

LSC · 13476-14160 · 685 bp

Back to Pteris

Pi 0.125
MarkerSeek score 84.60
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
685
variable_sites
254
pi
0.125
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TTCCGTGAAAGCCAAACTCA CTTTACCCGCTGTGACTACC 45.0 55.0 58.0 58.0 1455-1468 bp 0.857143 81.3
atpH-atpI_p2 CCCCGCCAATTTGGAAATTC CTTTACCCGCTGTGACTACC 50.0 55.0 58.0 58.0 1632 bp 0.142857 45.5
atpH-atpI_p3 CCCCGCCAATTTGGAAATTC AATTGGTCAGGTGCTTCGTT 50.0 45.0 58.0 58.0 1459 bp 0.142857 45.4
atpH-atpI_p4 CCCCGCCAATTTGGAAATTC ATTGGTCAGGTGCTTCGTTT 50.0 45.0 58.0 58.0 1458 bp 0.142857 45.4
atpH-atpI_p5 CCCCGCCAATTTGGAAATTC ATTTTGGCTGACGAGTTGGT 50.0 45.0 58.0 58.0 1204 bp 0.142857 45.4