Hotspot

rps4

Genome · 44084-44671 · 588 bp

Back to Pteridium

Pi 0.0068

MarkerSeek score 62.00

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 588
variable_sites: 4
pi: 0.0068
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TCACTACGAGCCCTTTACCT	TTTTTACGTCTCGCTACCGA	50.0	45.0	57.8	57.0	705 bp	1.0	70.7
rps4_p2	TCACTACGAGCCCTTTACCT	GTTTTTACGTCTCGCTACCG	50.0	50.0	57.8	57.0	706 bp	1.0	70.4
rps4_p3	CACTACGAGCCCTTTACCTG	TTTTTACGTCTCGCTACCGA	55.0	45.0	57.7	57.0	704 bp	1.0	70.2
rps4_p4	CACTACGAGCCCTTTACCTG	GTTTTTACGTCTCGCTACCG	55.0	50.0	57.7	57.0	705 bp	1.0	69.8
rps4_p5	TCACTACGAGCCCTTTACCT	AGGAGTTTTTACGTCTCGCT	50.0	45.0	57.8	56.9	710 bp	1.0	69.8