Hotspot

chlL

Genome · 128131-129012 · 882 bp

Back to Pteridium

Pi 0.0034

MarkerSeek score 60.70

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 882
variable_sites: 3
pi: 0.0034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	ACGAAAATGCAGCCTCTTCA	GTCGGGAGAAATGAACACGA	45.0	50.0	58.1	57.9	941 bp	1.0	78.8
chlL_p2	ACGAAAATGCAGCCTCTTCA	AGTCGGGAGAAATGAACACG	45.0	50.0	58.1	57.9	942 bp	1.0	78.8
chlL_p3	CACGAAAATGCAGCCTCTTC	GTCGGGAGAAATGAACACGA	50.0	50.0	57.7	57.9	942 bp	1.0	77.6
chlL_p4	CACGAAAATGCAGCCTCTTC	AGTCGGGAGAAATGAACACG	50.0	50.0	57.7	57.9	943 bp	1.0	77.6
chlL_p5	ACACGAAAATGCAGCCTCTT	GTCGGGAGAAATGAACACGA	45.0	50.0	58.4	57.9	943 bp	1.0	76.5