Hotspot

trnN-GUU-ycf2

Genome · 129522-130100 · 579 bp

Back to Pteridium

Pi 0.00518

MarkerSeek score 61.30

Primer available yes

Label trnN-GUU-ycf2

Feature Metadata

label_name: trnN-GUU-ycf2
feature_type: igs
region: Genome
length_bp: 579
variable_sites: 3
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf2_p1	CCCTACCACTTTCCTCGGTA	GCGTCACTGGATCGAACAA	55.0	52.6	58.1	58.2	701 bp	1.0	69.8
trnN-GUU-ycf2_p2	TGGTCGTAGGTTCGAATCCT	GCGTCACTGGATCGAACAA	50.0	52.6	58.2	58.2	648 bp	1.0	69.7
trnN-GUU-ycf2_p3	TACCACTTTCCTCGGTAGCT	GCGTCACTGGATCGAACAA	50.0	52.6	57.8	58.2	698 bp	1.0	69.1
trnN-GUU-ycf2_p4	CCCTACCACTTTCCTCGGTA	CGTCACTGGATCGAACAACG	55.0	55.0	58.1	59.3	700 bp	1.0	68.9
trnN-GUU-ycf2_p5	CGATTGGTCGTAGGTTCGAA	GCGTCACTGGATCGAACAA	50.0	52.6	57.7	58.2	652 bp	1.0	68.8