Hotspot

trnL-UAG-ccsA

Genome · 111851-112123 · 273 bp

Back to Pteridium

Pi 0.00375
MarkerSeek score 41.80
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
Genome
length_bp
273
variable_sites
1
pi
0.00375
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 CTCTTAGGAAGCAGTGCCAG AAAAACCCGTTGTGCAAAGG 55.0 45.0 58.0 58.0 480-486 bp 1.0 81.0
trnL-UAG-ccsA_p2 CTTAGGAAGCAGTGCCAGAG AAAAACCCGTTGTGCAAAGG 55.0 45.0 58.0 58.0 478-484 bp 1.0 81.0
trnL-UAG-ccsA_p3 CTCTTAGGAAGCAGTGCCAG AGTAGCAAAGGCATGAGTCG 55.0 50.0 58.0 58.0 656-662 bp 1.0 80.8
trnL-UAG-ccsA_p4 CTTAGGAAGCAGTGCCAGAG AGTAGCAAAGGCATGAGTCG 55.0 50.0 58.0 58.0 654-660 bp 1.0 80.8
trnL-UAG-ccsA_p5 CTTAGGAAGCAGTGCCAGAG AAGATCCGCACAACAAGGTT 55.0 45.0 58.0 58.0 775-781 bp 1.0 80.6