Hotspot

ycf2-trnN-GUU

Genome · 106598-107176 · 579 bp

Back to Pteridium

Pi 0.00518

MarkerSeek score 61.30

Primer available yes

Label ycf2-trnN-GUU

Feature Metadata

label_name: ycf2-trnN-GUU
feature_type: igs
region: Genome
length_bp: 579
variable_sites: 3
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnN-GUU_p1	GCGTCACTGGATCGAACAA	CCCTACCACTTTCCTCGGTA	52.6	55.0	58.2	58.1	701 bp	1.0	69.8
ycf2-trnN-GUU_p2	GCGTCACTGGATCGAACAA	TGGTCGTAGGTTCGAATCCT	52.6	50.0	58.2	58.2	648 bp	1.0	69.7
ycf2-trnN-GUU_p3	GCGTCACTGGATCGAACAA	TACCACTTTCCTCGGTAGCT	52.6	50.0	58.2	57.8	698 bp	1.0	69.1
ycf2-trnN-GUU_p4	CGTCACTGGATCGAACAACG	CCCTACCACTTTCCTCGGTA	55.0	55.0	59.3	58.1	700 bp	1.0	68.9
ycf2-trnN-GUU_p5	GCGTCACTGGATCGAACAA	CGATTGGTCGTAGGTTCGAA	52.6	50.0	58.2	57.7	652 bp	1.0	68.8