Hotspot

rpl14-rpl16

Genome · 78996-79398 · 403 bp

Back to Pteridium

Pi 0.0249

MarkerSeek score 54.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 403
variable_sites: 10
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CGCTGTTGTCTGCTACATCT	TCGAACCTGCTTGGATTACG	50.0	50.0	58.0	58.0	750-751 bp	1.0	81.2
rpl14-rpl16_p2	CGCTGTTGTCTGCTACATCT	ATGGAAATCTTCACGTCCGG	50.0	50.0	58.0	58.0	910-911 bp	1.0	80.9
rpl14-rpl16_p3	CGCTGTTGTCTGCTACATCT	GACAAATAGAAGCGGGGAGG	50.0	55.0	58.0	58.0	726-727 bp	1.0	80.9
rpl14-rpl16_p4	CGCTGTTGTCTGCTACATCT	GAATTCATGAGGAGCCGGTT	50.0	50.0	58.0	58.0	932-933 bp	1.0	80.7
rpl14-rpl16_p5	TACAACACCTCGGGAGCTAA	TCGAACCTGCTTGGATTACG	50.0	50.0	58.1	58.0	1075-1076 bp	1.0	80.3