Hotspot

rpl33-rps18

Genome · 65644-65715 · 72 bp

Back to Pteridium

Pi 0.0278

MarkerSeek score 64.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: Genome
length_bp: 72
variable_sites: 2
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AATCGCCGTAACACACCTAC	GAACGTCTACGGGAAGATCG	50.0	55.0	58.0	57.9	197 bp	1.0	80.0
rpl33-rps18_p2	AATCGCCGTAACACACCTAC	CTCACGAATCGGCGAAGTAA	50.0	50.0	58.0	58.1	263 bp	1.0	79.8
rpl33-rps18_p3	AATCGCCGTAACACACCTAC	TCACGAATCGGCGAAGTAAG	50.0	50.0	58.0	58.1	262 bp	1.0	79.8
rpl33-rps18_p4	AATCGCCGTAACACACCTAC	CGGCGAAGTAAGCTCGTATT	50.0	50.0	58.0	58.2	254 bp	1.0	79.3
rpl33-rps18_p5	AATCGCCGTAACACACCTAC	TCAGAGCGAATAGACGGTGA	50.0	50.0	58.0	58.3	215 bp	1.0	78.7