Hotspot

petA-psbJ

Genome · 61385-62373 · 989 bp

Back to Pteridium

Pi 0.00506

MarkerSeek score 61.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 989
variable_sites: 5
pi: 0.00506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGTTGTTTTGGCACAGATC	GCCGGTACACTTGTGATAGG	50.0	55.0	57.9	58.1	1147-1149 bp	1.0	79.4
petA-psbJ_p2	CGGTTGTTTTGGCACAGATC	ACTCAGGGTTAGGGTCATCC	50.0	55.0	57.9	58.1	1098-1100 bp	1.0	79.0
petA-psbJ_p3	TGGCACAGATCTTCCTTGTG	GCCGGTACACTTGTGATAGG	50.0	55.0	57.8	58.1	1138-1140 bp	1.0	78.3
petA-psbJ_p4	CGGTTGTTTTGGCACAGATC	ACGGAGCTTACTCAGGGTTA	50.0	50.0	57.9	57.8	1107-1109 bp	1.0	78.1
petA-psbJ_p5	CGGTTGTTTTGGCACAGATC	TACGGAGCTTACTCAGGGTT	50.0	50.0	57.9	57.8	1108-1110 bp	1.0	78.1