Hotspot

trnG-UCC-trnR-TCT

Genome · 9913-10058 · 146 bp

Back to Pteridium

Pi 0.0274

MarkerSeek score 68.30

Primer available yes

Label trnG-UCC-trnR-TCT

Feature Metadata

label_name: trnG-UCC-trnR-TCT
feature_type: igs
region: Genome
length_bp: 146
variable_sites: 4
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-TCT_p1	TGGGTTCCCGTCGACTATAA	GCTTAGTCGCCTCAAAGACA	50.0	50.0	57.9	57.9	311 bp	1.0	79.2
trnG-UCC-trnR-TCT_p2	TACTGATGCGGGTTCGATTC	GCTTAGTCGCCTCAAAGACA	50.0	50.0	57.8	57.9	274 bp	1.0	78.8
trnG-UCC-trnR-TCT_p3	CTACTGATGCGGGTTCGATT	GCTTAGTCGCCTCAAAGACA	50.0	50.0	57.8	57.9	275 bp	1.0	78.7
trnG-UCC-trnR-TCT_p4	TTCTGAATTAGCGGCGTCAT	GCTTAGTCGCCTCAAAGACA	45.0	50.0	57.7	57.9	336 bp	1.0	77.8
trnG-UCC-trnR-TCT_p5	AATTAGCGGCGTCATAACCA	GCTTAGTCGCCTCAAAGACA	45.0	50.0	57.7	57.9	331 bp	1.0	77.8