Hotspot

trnS-GCU-ycf12

Genome · 7993-8677 · 685 bp

Back to Pteridium

Pi 0.00584

MarkerSeek score 62.10

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 685
variable_sites: 4
pi: 0.00584
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	TTTAGACCTCTCGGCCATCT	TGCCAAAACAGCAAGTTGTG	50.0	45.0	57.8	58.0	748-752 bp	1.0	79.1
trnS-GCU-ycf12_p2	TTTAGACCTCTCGGCCATCT	ATGCCACTACCAATGCCAAA	50.0	45.0	57.8	58.1	761-765 bp	1.0	78.6
trnS-GCU-ycf12_p3	TTTAGACCTCTCGGCCATCT	GCCACTACCAATGCCAAAAC	50.0	50.0	57.8	57.9	759-763 bp	1.0	78.5
trnS-GCU-ycf12_p4	ATCCGACGCTTTAGACCTCT	TGCCAAAACAGCAAGTTGTG	50.0	45.0	58.2	58.0	757-761 bp	1.0	78.5
trnS-GCU-ycf12_p5	TTTAGACCTCTCGGCCATCT	CGGGGATGAGGTCACAAATT	50.0	50.0	57.8	57.9	822-826 bp	1.0	78.1