Hotspot

rpl16

LSC · 82039-83491 · 1453 bp

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Pi 0.0346

MarkerSeek score 74.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1453
variable_sites: 45
pi: 0.0346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCAATTTTCCAAGACCCCCT	AAGCTAAACCCATTCATTCT	50.0	35.0	58.4	52.4	1475-1605 bp	1.0	46.3
rpl16_p2	GCAATTTTCCAAGACCCCCT	AGCTAAACCCATTCATTCTT	50.0	35.0	58.4	52.4	1474-1604 bp	1.0	46.3
rpl16_p3	GCAATTTTCCAAGACCCCCT	AAAGCTAAACCCATTCATTCT	50.0	33.3	58.4	53.3	1476-1606 bp	1.0	46.3
rpl16_p4	GCAATTTTCCAAGACCCCCT	AAGCTAAACCCATTCATTCTT	50.0	33.3	58.4	53.3	1475-1605 bp	1.0	46.3
rpl16_p5	GCAATTTTCCAAGACCCCCT	AGCTAAACCCATTCATTCTTT	50.0	33.3	58.4	53.3	1474-1604 bp	1.0	46.3