Hotspot

ndhA

SSC · 119968-122303 · 2336 bp

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Pi 0.0235

MarkerSeek score 56.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2336
variable_sites: 55
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTTGACCAATGTACCTGGCA	GTCCGCCAGGGTTTATCAAT	50.0	50.0	57.8	57.9	2619-2678 bp	1.0	79.8
ndhA_p2	TTGACCAATGTACCTGGCAG	GTCCGCCAGGGTTTATCAAT	50.0	50.0	57.8	57.9	2618-2677 bp	1.0	79.8
ndhA_p3	ACCGACCCAACGAAATTTGA	GTCCGCCAGGGTTTATCAAT	45.0	50.0	58.0	57.9	2979 bp	0.5	59.3
ndhA_p4	GGTAACCGACCCAACGAAAT	GTCCGCCAGGGTTTATCAAT	50.0	50.0	58.2	57.9	2983 bp	0.5	58.1
ndhA_p5	TTGACCAATGTACCTGGCAG	TAATAGGGGATCGGGGCATT	50.0	50.0	57.8	57.9	2660 bp	0.5	58.0