Hotspot

rpl32-trnL-UAG

SSC · 113845-114519 · 675 bp

Back to Psychotria

Pi 0.0609
MarkerSeek score 80.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
675
variable_sites
39
pi
0.0609
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTGTACAGCAAACAACTAAA AGCAGCGTGTCTACCAATTT 28.6 45.0 52.1 57.8 711-731 bp 1.0 48.2
rpl32-trnL-UAG_p2 TTTTGTACAGCAAACAACTAA AGCAGCGTGTCTACCAATTT 28.6 45.0 52.1 57.8 712-732 bp 1.0 48.2
rpl32-trnL-UAG_p3 TTTGTACAGCAAACAACTAAA TCCTAAGAGCAGCGTGTCTA 28.6 50.0 52.1 57.9 718-738 bp 1.0 48.1
rpl32-trnL-UAG_p4 TTTTGTACAGCAAACAACTAA TCCTAAGAGCAGCGTGTCTA 28.6 50.0 52.1 57.9 719-739 bp 1.0 48.1
rpl32-trnL-UAG_p5 TTTTTGTACAGCAAACAACTA TCCTAAGAGCAGCGTGTCTA 28.6 50.0 52.1 57.9 720-740 bp 1.0 48.1