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rps8-rpl14

LSC · 81388-81543 · 156 bp

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Pi 0.0467

MarkerSeek score 69.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 7
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CTCGGCAATAGTATCCCTGC	TTTCATTAGCTCCCGAGGTA	55.0	45.0	57.9	55.6	200-206 bp	1.0	65.6
rps8-rpl14_p2	CTCGGCAATAGTATCCCTGC	TCATTAGCTCCCGAGGTATT	55.0	45.0	57.9	55.4	198-204 bp	1.0	63.8
rps8-rpl14_p3	CTCGGCAATAGTATCCCTGC	TTCATTAGCTCCCGAGGTAT	55.0	45.0	57.9	55.4	199-205 bp	1.0	63.7
rps8-rpl14_p4	CTCGGCAATAGTATCCCTGC	TTCATTAGCTCCCGAGGTATT	55.0	42.9	57.9	56.2	199-205 bp	1.0	62.2
rps8-rpl14_p5	CTCGGCAATAGTATCCCTGC	TTTCATTAGCTCCCGAGGTAT	55.0	42.9	57.9	56.2	200-206 bp	1.0	62.2